Description
Cell to transfect:
Cell Name:® Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA)
Cell Synonyms: : HFLS, HFLS-OA, HFLS-RA, synoviocyte
Human Fibroblast-like synoviocytes (HFLS) represent a specialised cell type located inside joints in the synovium. HFLS are long known for their role in joint destruction by producing cytokines and metalloproteinases that contribute to cartilage degradation. Proinflammatory cytokines induce the proliferation, collagenase and aggrecanase production and GM-CSF secretion on HFLS. Ongoing arthritis research also has shown that HFLS express apoptosis and P53 mutations
Human Fibroblast-Like Synoviocytes (HFLS) are usually isolated from normal synovial tissues. These cells can be used as a good in vitro model for studying the pathogenesis of chronic inflammatory diseases, such as rheumatoid arthritis.
The Transfection Reagent Features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human fibroblast-like synoviocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human fibroblast-like synoviocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Fibroblast-Like Synoviocyte Avalanche Transfection Reagent.
FIG. 2. Human fibroblast-like synoviocytes were transfected with GFP vector (pEGFP-N3) by using Human Fibroblast-Like Synoviocyte Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
BALB/3T3 clone A31
3T3-Swiss albino Cell
CT26.WT Cell
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
Weight | 0.5 lbs |
---|---|
Adherence Phenotype |
Adherent |
Cell Type |
Synoviocyte |
Disease |
Healthy |
Names starting from |
H |
Primary/Cell Line |
Primary Cell |
Product Sizes |
0.5 ml, 1.5 ml |
Species |
Human |
Tissue Sources |
Synovial Tissue |
Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
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