EZT-HC00-1 Protocol.
Description
Cell to transfect:
Cell Name: Human Chondrocyte (HC, NHAC, HC-RA, HC-OA)
Cell Synonyms: HC, NHAC, HC-RA, HC-OA
Chondrocytes produce and maintain the extracellular matrix of cartilage. Human Chondrocytes are usually derived from normal human articular cartilage. Human Chondrocytes growing in serial monolayer culture lose their phenotypic markers and became de-differentiated. This de-differentiation stage can be reversed by culturing them in semi-solid gel. Human Chondrocyte has served as a useful model for studying chondrocyte differentiation and the effects of cytokines and growth factors on the suppression and maintenance of differentiated cartilage phenotype.
Applications:
- Osteoarthritis
- Cartilage failure
- Cellular matrix linkage rupture
- Chondrocyte protein synthesis inhibition
- Chondrocyte apoptosis
The Transfection Reagent Features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Unique formulation-maximize transfection performance in HC.
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human chondrocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human chondrocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Chondrocyte Avalanche Transfection Reagent.
For Other Cells
Human Chondrocyte (HC, NHAC, HC-RA, HC-OA) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
WI-38 Cell
MRC-5 Cell
LNCaP Cell
DU 145 Cell
22Rv1 Cell
VCaP Cell
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Chondrocyte |
| Disease |
Healthy |
| Names starting from |
H |
| Primary/Cell Line |
Primary Cell |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Cartilage |
| Subcategories |
Cell Type/Cell Line Specific |
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