EZT-HCF0-1 Protocol.
Description
Cell to transfect:
Cell Name:® Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF)
Cell Synonyms: : Cardiac Fibroblasts, HCF, NHCF-A, NHCF-V, AoAF
Human Cardiac Fibroblasts (HCF, NHCF-A and -V) are derived from normal human heart tissues (atrium or ventricle). Aortic Adventitial Fibroblasts (AoAF) (isolated from the tunica external of the ascending or descending aorta) are Cardiac Related fibroblasts . Human Cardiac fibroblasts or Cardiac Related Fibroblasts have been used to study the effects of TNF-alpha and angiotensin (Ang) II levels on fibroblast function. Expression of MyoD can induce myogenesis of cardiac fibroblasts. Cardiac fibroblasts involved in aortic regurgitation synthesize more Fibronectin. Estradiol inhibits cardiac fibroblast growth through conversion to methoxyestradiols, which may protect against remodeling involved in heart disease. TGF-beta stimulates differentiation of fibroblasts to myofibroblasts, which increases collagen production.
Applications:
- Vascular diseases
- Neointimal formation
- Vascular remodeling
- Atherosclerosis
- Hypertension
- Myocardial repair
- Extracellular maintenance during cardiac cell / tissue growth
- Cardiac Fibrosis Paracrine signalling
- Toxicology
- Gene expression
The Transfection Reagent Features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Unique formulation-maximize transfection performance in Human Cardiac fibroblasts and Cardiac Related Fibroblasts.
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human dermal fibroblasts after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human dermal fibroblasts. This test result was confirmed with repeat experiments. The candidate formulas were also tested in human cardiac fibroblasts by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Cardiac Fibroblast Avalanche Transfection Reagent.
FIG. 2. Human Cardiac Fibroblasts were transfected with GFP vector (pEGFP-N3) by using Human Cardiac Fibroblast Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Fibroblast |
| Disease |
Healthy |
| Names starting from |
H |
| Primary/Cell Line |
Primary Cell |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Heart |
| Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
