Human Artery Endothelial Cell Avalanche® Transfection Reagent

Name: Human Artery Endothelial Cell Avalanche® Transfection Reagent
SKU: EZT-HAOE-1
Availability: InStock

Transfection of primary cells, such as Human Artery Endothelial Cell, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

Human Artery Endothelial Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Artery Endothelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Artery Endothelial Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Artery Endothelial Cell Avalanche® Transfection Reagent for transfection on Human Artery Endothelial Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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SKU: EZT-HAOE-1 Categories: Cell Type/Cell Line-specific Transfection Reagent, Endothelial Cell Transfection, For Primary Cells, Transfection Reagents Tags: Aortic, Artery, Brachiocephalic, Carotid, Coronary, endothelial cell, HAOEC, HBcAEC, HCAEC, HCtAEC, HITAEC, HPAEC, HScAEC, Internal Thoracic, Pulmonary, Subclavian

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$714.00 – $1,264.00

Description

Cell to transfect:

Cell Name: Human Artery Endothelial Cell

Cell Synonyms: HAOEC, HBcAEC, HCtAEC, HCAEC, HITAEC, HPAEC, HScAEC, Aortic, Brachiocephalic, Carotid, Coronary, Internal Thoracic, Pulmonary, Subclavian

Vascular endothelial cells line the entire circulatory system, from the heart to the smallest capillaries. These cells have very distinct and unique functions that are paramount to vascular biology. These cells also play a pivotal role in a diverse range of physiological activities, such as angiogenesis, forming the selective blood barrier, arterial diseases and cancer development.

The Transfection Reagent Features:

Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into Human Artery Endothelial Cells
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Compatible with serum
Compatible with transfection in any plate formats
Developed and manufactured by EZ Biosystems

The transfection reagent work best for the following Human Artery Endothelial cells:

Human Aortic Endothelial Cell,
Human Brachiocephalic Artery Endothelial Cell,
Human Carotid Artery Endothelial Cell,
Human Coronary Artery Endothelial Cell,
Human Internal Artery Thoracic Endothelial Cell,
Human Pulmonary Artery Endothelial Cell,
Human Subclavian Artery Endothelial Cell

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human aortic endothelial cell after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human aortic endothelial cell. This test result was also confirmed by transfecting other artery endothelial cells mentioned above. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Artery Endothelial Cell Avalanche Transfection Reagent.

For Other Cells

Human Artery Endothelial Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight	0.5 lbs
Adherence Phenotype	Adherent
Cell Type	Endothelial Cell
Disease	Healthy
Names starting from	H
Primary/Cell Line	Primary Cell
Product Sizes	0.5 ml, 1.5 ml
Species	Human
Tissue Sources	Artery
Subcategories	Cell Type/Cell Line Specific

Documents

Protocols

EZT-HAOE-1 Protocol.

MSDS

EZT-HAOE-1 MSDS

Citations or Feedback

Samokhin AO, Stephens T, Wertheim BM, Wang RS, Vargas SO, Yung LM, Cao M, Brown M, Arons E, Dieffenbach PB, Fewell JG, Matar M, Bowman FP, Haley KJ, Alba GA, Marino SM, Kumar R, Rosas IO, Waxman AB, Oldham WM, Khanna D, Graham BB, Seo S, Gladyshev VN, Yu PB, Fredenburgh LE, Loscalzo J, Leopold JA, Maron BA. NEDD9 targets COL3A1 to promote endothelial fibrosis and pulmonary arterial hypertension. Sci Transl Med. 2018 Jun 13;10(445). doi: 10.1126/scitranslmed.aap7294. PubMed PMID: 29899023; PubMed Central PMCID: PMC6223025.