EZT-HBEC-1 Protocol.
Description
Cell to transfect:
Cell Name: Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC)
Cell Synonyms: : HBEpC, HTEpC, NHBE, SAEC
Airway epithelial cells are targets in asthma, viral infection and fibrotic lung disease. Airway epithelial cells include human Bronchial, Tracheal, and Small Airway Epithelial Cells. These cells have been used to study the regulation of intracellular pH, the stimulant effect of IL-1b on airway epithelial cell growth, and the effect of human rhinovirus infection in terms of upregulation of ICAM-1 expression on epithelial cells.
Applications:
- Asthma
- COPD
- Airborne viruses
- Allergies
- Inhalation of nanoparticles
- Cancer
- Cystic Fibrosis
- DoD studies
The Transfection Reagent Features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into Human Airway Epithelial Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human bronchial epithelial cells (HBEpC) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human bronchial epithelial cells (HBEpC). This test result was also confirmed by transfecting other airway epithelial Cell mentioned above. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Airway Epithelial Cell Avalanche Transfection Reagent.
For Other Cells
Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Epithelial Cell |
| Disease |
Healthy |
| Names starting from |
H |
| Primary/Cell Line |
Primary Cell |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Bronchia |
| Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
- Zhou, Y., Li, P., Goodwin, A. J., Cook, J. A., Halushka, P. V., Chang, E., . . . Fan, H. (2019). Exosomes from endothelial progenitor cells improve outcomes of the lipopolysaccharide-induced acute lung injury. Crit Care, 23(1), 44. doi:10.1186/s13054-019-2339-3
- Oniki, K., Nohara, H., Nakashima, R., Obata, Y., Muto, N., Sakamoto, Y., . . . Saruwatari, J. (2020). The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties. Sci Rep, 10(1), 5973. doi:10.1038/s41598-020-62872-5 EZT-HBEC-1 plasmids on Primary normal human bronchial epithelial (NHBE) cells
