HL-60 Cell Avalanche® Transfection Reagent (human leukemia cell)

Transfection of suspension cells, such as HL-60 cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation..

HL-60 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HL-60 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HL-60 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

$714.00 – $1,264.00

& Free Shipping
SKU: EZT-HL60-1 Categories: , , , , Tags: , , , , , , , , ,

Request Quote

$714.00 – $1,264.00

 
Description

Cell to transfect:

Designations: HL-60

Cell Synonyms: HL60, CCL-240, promyelocytic leukemia, promyeloblast, myeloblastic, NCI60, NCI-60, human leukemia cell

Organism: Homo sapiens (human)

Tissue: peripheral blood; acute promyelocytic leukemia; promyeloblast

Age: 36 years

Gender: female

Ethnicity: Caucasian

Morphology: myeloblastic

Growth properties: suspension

Tumorigenic: yes, form colonies in semi-solid media and produce subcutaneous myeloid tumors in nude mice.

Oncogene: myc +

Receptors expressed: complement; Fc

Products: tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid

Depositors: R.C. Gallo

HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. The line is positive for myc oncogene expression.

HL-60 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in HL-60 Cells.
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HL-60 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HL-60 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HL-60 Cell Avalanche Transfection Reagent.

For Other Cells

HL-60 Cell Avalanche® Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies.

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

MG-63 Cell

WI-38 Cell

COS-7 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Suspension
Cell Type

Others
Disease

Cancer
Names starting from

H
Primary/Cell Line

Cell Line
Product Sizes

0.5 ml, 1.5 ml
Species

Human
Tissue Sources

Blood
Subcategories

Cell Type/Cell Line Specific

Documents


Protocols



PDF icon imageEZT-HL60-1 Protocol.





MSDS



PDF icon imageEZT-HL60-1 MSDS





Citations or Feedback

  • Li, Y., Li, M., Weigel, B., Mall, M., Werth, V. P., & Liu, M. L. (2020). Nuclear envelope rupture and NET formation is driven by PKCalpha-mediated lamin B disassembly. EMBO Rep, 21(8), e48779. doi:10.15252/embr.201948779
  • Taylor, S., Isobe, S., Cao, A., Contrepois, K., Benayoun, B. A., Jiang, L., . . . Rabinovitch, M. (2022). Endogenous Retroviral Elements Generate Pathologic Neutrophils in Pulmonary Arterial Hypertension. Am J Respir Crit Care Med. doi:10.1164/rccm.202102-0446OC

Testimonials and Citations

 
Description

Cell to transfect:

Designations: HL-60

Cell Synonyms: HL60, CCL-240, promyelocytic leukemia, promyeloblast, myeloblastic, NCI60, NCI-60, human leukemia cell

Organism: Homo sapiens (human)

Tissue: peripheral blood; acute promyelocytic leukemia; promyeloblast

Age: 36 years

Gender: female

Ethnicity: Caucasian

Morphology: myeloblastic

Growth properties: suspension

Tumorigenic: yes, form colonies in semi-solid media and produce subcutaneous myeloid tumors in nude mice.

Oncogene: myc +

Receptors expressed: complement; Fc

Products: tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid

Depositors: R.C. Gallo

HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. The line is positive for myc oncogene expression.

HL-60 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in HL-60 Cells.
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HL-60 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HL-60 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HL-60 Cell Avalanche Transfection Reagent.

For Other Cells

HL-60 Cell Avalanche® Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies.

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

MG-63 Cell

WI-38 Cell

COS-7 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Suspension
Cell Type

Others
Disease

Cancer
Names starting from

H
Primary/Cell Line

Cell Line
Product Sizes

0.5 ml, 1.5 ml
Species

Human
Tissue Sources

Blood
Subcategories

Cell Type/Cell Line Specific

Documents


Protocols



PDF icon imageEZT-HL60-1 Protocol.





MSDS



PDF icon imageEZT-HL60-1 MSDS





Citations or Feedback

  • Li, Y., Li, M., Weigel, B., Mall, M., Werth, V. P., & Liu, M. L. (2020). Nuclear envelope rupture and NET formation is driven by PKCalpha-mediated lamin B disassembly. EMBO Rep, 21(8), e48779. doi:10.15252/embr.201948779
  • Taylor, S., Isobe, S., Cao, A., Contrepois, K., Benayoun, B. A., Jiang, L., . . . Rabinovitch, M. (2022). Endogenous Retroviral Elements Generate Pathologic Neutrophils in Pulmonary Arterial Hypertension. Am J Respir Crit Care Med. doi:10.1164/rccm.202102-0446OC

Testimonials and Citations

Related products

Shopping Cart
Scroll to Top