HepG2/C3A Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HepG2/C3A cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HepG2/C3A cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Comments: C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium. As the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion. Gluconeogenesis activity is strongly oxgen dependent. The cells have nitrogen metabolizing activity comparable to perfused rat livers.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in HepG2/C3A cells.
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Efficient for plasmid DNA/siRNA co-transfection
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HEP G2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HEP G2 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on HepG2/C3A cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as HepG2/C3A Cell Avalanche Transfection Reagent.
FIG. 2. HepG2/C3A cells were transfected with GFP vector (pEGFP-N3) by using HepG2/C3A Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
HepG2/C3A Cell Avalanche® Transfection Reagent (human hepatocellular carcinoma cell) can also be used on the following cells with high transfection efficiencies.
Hepa 1-6 Cell
Hep G2 Cell
Hep 3B cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
CHO-K1 Cell
SK-OV-3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Comments: C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium. As the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion. Gluconeogenesis activity is strongly oxgen dependent. The cells have nitrogen metabolizing activity comparable to perfused rat livers.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in HepG2/C3A cells.
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Efficient for plasmid DNA/siRNA co-transfection
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HEP G2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HEP G2 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on HepG2/C3A cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as HepG2/C3A Cell Avalanche Transfection Reagent.
FIG. 2. HepG2/C3A cells were transfected with GFP vector (pEGFP-N3) by using HepG2/C3A Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
HepG2/C3A Cell Avalanche® Transfection Reagent (human hepatocellular carcinoma cell) can also be used on the following cells with high transfection efficiencies.
Hepa 1-6 Cell
Hep G2 Cell
Hep 3B cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
CHO-K1 Cell
SK-OV-3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
EZT-C3A0-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HEP G2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HEP G2 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on HepG2/C3A cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as HepG2/C3A Cell Avalanche Transfection Reagent.
