Hep 3B Cell Avalanche® Transfection Reagent (human hepatocellular carcinoma cell)
Hep 3B Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on Hep 3B cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Hep 3B cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Hep 3B Cell Avalanche® Transfection Reagent for transfection on Hep 3B cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into Hep 3B cells.
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Hep G2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Hep G2 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on Hep 3B cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Hep 3B Cell Avalanche Transfection Reagent.
FIG. 2. Hep 3B cells were transfected with GFP vector (pEGFP-N3) by using Hep 3B Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
Hep 3B Cell Avalanche® Transfection Reagent (human hepatocellular carcinoma cell) can also be used on the following cells with high transfection efficiencies.
Hepa 1-6 Cell
Hep G2 Cell
HepG2/C3A Cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
CHO-K1 Cell
SK-OV-3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into Hep 3B cells.
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Hep G2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Hep G2 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on Hep 3B cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Hep 3B Cell Avalanche Transfection Reagent.
FIG. 2. Hep 3B cells were transfected with GFP vector (pEGFP-N3) by using Hep 3B Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
Hep 3B Cell Avalanche® Transfection Reagent (human hepatocellular carcinoma cell) can also be used on the following cells with high transfection efficiencies.
Hepa 1-6 Cell
Hep G2 Cell
HepG2/C3A Cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
CHO-K1 Cell
SK-OV-3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
EZT-HEP3-1 Protocol.
