HCT-15 Cell Avalanche® Transfection Reagent

 
Description

Cells to transfect:

Cell Line name: HCT-15 (ATCC® CCL-225®)

Cell Synonyms: ATCC® CCL-225®, HCT15, HCT.15, NCI60, NCI-60

Organism: Homo sapiens, human

Tissue Source: colon

Morphology: epithelial

Culture Properties: adherent

Disease: Dukes’ type C, colorectal adenocarcinoma

Gender: male

Derivation: Evidence from DNA fingerprinting indicates that this line and DLD-1 (ATCC CCL-221) are derived from the same individual; however, isoenzymology and cytogenetic data leave some doubt.

Genes Expressed: carcinoembryonic antigen (CEA) 5.4 ng/106 cells/10 days. The cells are positive for keratin by immunoperoxidase staining.

Tumorigenic: Yes

Comments: HCT-15 cells are CSAp negative (CSAp-). The cells are positive for keratin by immunoperoxidase staining.

Applications: This cell line is a suitable transfection host.

HCT-15 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in HCT-15 cells
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Active in serum-containing medium
• Suitable for Reverse Transfection
• Compatible with transfection in any plate format
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HCT-15 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HCT-15 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HCT-15 Cell Avalanche® Transfection Reagent.

For Other Cells

HCT-15 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

HCC2998 Cell

KM12 Cell

T84 Cell

DLD-1 Cell

HT-29 Cell

Caco-2 Cell

LoVo Cell

SW480 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

MSDS

EZT-HCT1-2 MSDS

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