HCC1937 Cell Avalanche® Transfection Reagent (human breast carcinoma cell)

 
Description

Cell to transfect:

Designations: HCC1937

Cell Synonyms: : CRL-2336, human breast carcinoma cell

Depositors: AF Gazdar, AK Virmani

Growth Properties: adherent, The line grows as large epithelial cells with a tendency to float at high cell densities

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tissue: duct

Tumor Stage: TNM stage IIB, grade 3

Disease: primary ductal carcinoma

Cellular Products: Epithelial glycoprotein 2 (EGP2), cytokeratin 19

Isolation date: October 13, 1995

progesterone receptor, negative

Oncogene: BRCA1 (mutated, insertion C at nucleotide 5382), her2/neu -, p53 -.

Age: 23 years adult

Gender: female

Ethnicity: Caucasian

Comments: This cell line was initiated from a primary ductal carcinoma on October 13, 1995, and took 11.5 months to establish. The cell line has an acquired homozygous deletion of the PTEN gene, and loss of heterozygosity at multiple loci known to be involved in the pathogenesis of breast cancer.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in HCC1937 cells.
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on ZR-75-1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for ZR-75-1 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on HCC1937 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as HCC1937 Cell Avalanche® Transfection Reagent.

FIG. 2. HCC1937 cells were transfected with GFP vector (pEGFP-N3) by using HCC1937 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells

HCC1937 Cell Avalanche® Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies.

4T1 Cell

ZR-75-1 Cell

BT-20 Cell

MDA-MB-453 Cell

MDA-MB-468 Cell

SK-BR-3 Cell

MDA-MB-231 Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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