EZT-H9C2-1 Protocol.
Description
Cell to transfect:
Designations: H9c2(2-1)
Cell Synonyms: : CRL-1446
Depositors: W Carlisle
Growth Properties: adherent
Organism: Rattus norvegicus
Morphology: myoblast
Source: heart
Strain: BD1X
Tissue: myocardium
Cellular Products: myokinase; creatine phosphokinase; myosin
Applications: transfection host
Receptors: acetylcholine, expressed
Age: embryo
H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle. Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation. Fusion occurs faster if the serum concentration in the medium is reduced to one percent.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Unique formulation-maximize transfection performance in H9c2(2-1) cells.
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Compatible with serum
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on H9c2(2-1) cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for H9c2(2-1) cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this H9c2(2-1) Cell Avalanche Transfection Reagent.
For Other Cells
H9c2(2-1) Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
L6 Cell
C2C12 Cell
786-O Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype | Adherent |
| Cell Type | Cardiac Cell |
| Disease | Healthy |
| Names starting from | H |
| Primary/Cell Line | Cell Line |
| Product Sizes | 0.5 ml, 1.5 ml |
| Species | Rat |
| Tissue Sources | Heart |
| Subcategories | Cell Type/Cell Line Specific |
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