COS-7 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on COS-7 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for COS-7 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of COS-7 Cell Avalanche® Transfection Reagent for transfection on COS-7 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Virus Susceptibility: SV40 (lytic growth); SV40 tsA209 at 40®C; SV40 mutants with deletions in the early region
Depositor: Y. Gluzman
This line was derived from the CV-1 cell line (ATCC® CCL-70®) by transformation with an origin defective mutant of SV40 which codes for wild-type T antigen.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in COS-7 cells.
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Efficient for plasmid DNA/siRNA co-transfection
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on COS-7 Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for COS-7 Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this COS-7 Cell Avalanche Transfection Reagent.
FIG. 2. COS-7 cells were transfected with GFP vector (pEGFP-N3) by using COS-7 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
COS-7 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
BJ Cell
Detroit 551 Cell
MG-63 Cell
WI-38 Cell
MRC-5 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Belkacemi, A., Beck, A., Wardas, B., Weissgerber, P., & Flockerzi, V. (2022). IP3-dependent Ca(2+) signals are tightly controlled by Cavbeta3, but not by Cavbeta1, 2 and 4. Cell Calcium, 104, 102573. doi:10.1016/j.ceca.2022.102573
Hofmann, L., Wang, H., Beck, A., Wissenbach, U., & Flockerzi, V. (2017). A conserved gating element in TRPV6 channels. Cell Calcium, 63, 24-28. doi:10.1016/j.ceca.2016.10.003
Peckys, D. B., Stoerger, C., Latta, L., Wissenbach, U., Flockerzi, V., & de Jonge, N. (2017). The stoichiometry of the TMEM16A ion channel determined in intact plasma membranes of COS-7 cells using liquid-phase electron microscopy. J Struct Biol, 199(2), 102-113. doi:10.1016/j.jsb.2017.05.009
Virus Susceptibility: SV40 (lytic growth); SV40 tsA209 at 40®C; SV40 mutants with deletions in the early region
Depositor: Y. Gluzman
This line was derived from the CV-1 cell line (ATCC® CCL-70®) by transformation with an origin defective mutant of SV40 which codes for wild-type T antigen.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in COS-7 cells.
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Efficient for plasmid DNA/siRNA co-transfection
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on COS-7 Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for COS-7 Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this COS-7 Cell Avalanche Transfection Reagent.
FIG. 2. COS-7 cells were transfected with GFP vector (pEGFP-N3) by using COS-7 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
COS-7 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
BJ Cell
Detroit 551 Cell
MG-63 Cell
WI-38 Cell
MRC-5 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Belkacemi, A., Beck, A., Wardas, B., Weissgerber, P., & Flockerzi, V. (2022). IP3-dependent Ca(2+) signals are tightly controlled by Cavbeta3, but not by Cavbeta1, 2 and 4. Cell Calcium, 104, 102573. doi:10.1016/j.ceca.2022.102573
Hofmann, L., Wang, H., Beck, A., Wissenbach, U., & Flockerzi, V. (2017). A conserved gating element in TRPV6 channels. Cell Calcium, 63, 24-28. doi:10.1016/j.ceca.2016.10.003
Peckys, D. B., Stoerger, C., Latta, L., Wissenbach, U., Flockerzi, V., & de Jonge, N. (2017). The stoichiometry of the TMEM16A ion channel determined in intact plasma membranes of COS-7 cells using liquid-phase electron microscopy. J Struct Biol, 199(2), 102-113. doi:10.1016/j.jsb.2017.05.009
EZT-COS7-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on COS-7 Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for COS-7 Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this COS-7 Cell Avalanche Transfection Reagent.
