EZT-BXPC-1 Protocol.
Description
Cell to transfect:
Designations: BxPC-3
Cell Synonyms: CRL-1687, human pancreas adenocarcinoma cell
Organism: Homo sapiens (human)
Tissue: pancreatic adenocarcinoma; cancer
Growth Properties: adherent
Morphology: epithelial
Age: 61 years
Gender: female
Products: mucin; pancreas cancer specific antigen (pancreas cancer associated antigen); carcinoembryonic antigen (CEA)
Tumorigenic: yes, Tumors developed within 21 days at 100%
frequency (5/5) in nude mice inoculated.
Depositors: M. H. Tan
The cells do not express the cystic fibrosis transmembrane conductance regulator (CFTR).
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Unique formulation-maximize transfection performance in BxPC-3 Cells.
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Capan-1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Capan-1 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on BxPC-3 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as BxPC-3 Cell Avalanche Transfection Reagent.
For Other Cells:
BxPC-3 Cell Avalanche® Transfection Reagent (human pancreas adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.
AR42J Cell
Capan-1 Cell
PANC-1 Cell
AsPC-1 Cell
293 Cell
293T/17 Cell
SK-OV-3 Cell
U-2 OS cell
Saos-2 Cell
SK-N-SH Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype | Adherent |
| Cell Type | Epithelial Cell |
| Disease | Cancer |
| Names starting from | B |
| Primary/Cell Line | Cell Line |
| Product Sizes | 0.5 ml, 1.5 ml |
| Species | Human |
| Tissue Sources | Pancreas |
| Subcategories | Cell Type/Cell Line Specific |
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