Beta-TC-6 Cell Avalanche® Transfection Reagent (mouse pancreatic cancer cell)

 
Description

Cell to transfect:

Designations: Beta-TC-6

Cell Synonyms: Beta-TC6, CRL-11506, mouse pancreatic cancer cell

Organism: Mus musculus (mouse, transgenic for SV40 large T antigen )

Tissue: pancreas; insulinoma; beta cell

Age: embryo

Morphology: epithelial

Growth properties: adherent

Depositors: CytoTherapeutics, Inc.

The cell line was derived from a pancreatic tumor(insulinoma) arising in a transgenic mouse. The mouse carried the pseudogene construct composed of the SV40 early region controlled by the rat insulin II gene promoter. The cells contain abundant insulin and small amounts of glucagon and somatostatin. They secrete insulin in response to glucose[PubMed: 7533732].

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in Beta-TC-6 cells.
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Compatible with serum
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on BETA-TC-6 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for BETA-TC-6 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this BETA-TC-6 Cell Avalanche Transfection Reagent.

For Other Cells

Beta-TC-6 Cell Avalanche® Transfection Reagent (mouse pancreatic cancer cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-BETA-1 Protocol.

MSDS

EZT-BETA-1 MSDS

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