Description
CRISPR-Cas9 mediated genome targeting:
CRISPR-Cas9 is a genome-editing technology adapted from a natural defense system in bacteria and archaea. In mammalian cells, a single-guide RNA (sgRNA) directs the Cas9 enzyme to a matching DNA sequence next to a compatible PAM sequence. Cas9 cuts the DNA, and the cell’s repair processes can then disrupt or alter the targeted gene.
Researchers use CRISPR-Cas9 to investigate gene function, model genomic changes involved in cancer and other diseases, and explore ways to correct disease-causing mutations. Successful editing depends on delivering the CRISPR components into the target cells and verifying the resulting DNA changes.
The Cas9 enzyme (green) generates breaks in double-stranded DNA by using its two catalytic centers to cleave each strand of a DNA target site next to a PAM sequence and matching the 20-nucleotide sequence of the single guide RNA (sgRNA).
The Transfection Reagent:
Features:
- Specifically designed for CRISPR-Cas9 gene editing transfection in mammalian cells
- Supports multiple CRISPR-Cas9 delivery formats: plasmid(s) encoding Cas9 and gRNA; a Cas9-encoding plasmid co-delivered with gRNA; Cas9 mRNA co-delivered with gRNA; or a preassembled Cas9–gRNA ribonucleoprotein (RNP) complex.
- Compatible with TrueGuide™ Synthetic gRNA, TrueCut™ Cas9 Protein v2, and CRISPR libraries from Thermo Fisher Scientific.
- Great for CRISPR-Cas9 gene editing in difficult-to-transfect cells, such as primary cells, suspension culture cells, or neural cells.
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases - Compatible with serum
- Chemically defined compounds and completely free of animal‐derived components. cells.
Data
Avalanche®-CRISPR outperforms other brands of transfection reagents on transferring Cas9/GFP co-expression plasmids into some difficult-to-transfect cells. GFP expression in different type of cells after transfecting pCas9-GFP plasmid (Addgene #44719) by using Avalanche®-CRISPR, Lipofectamine® 2000 (L2K), and Lipofectamine® 3000 (L3K). The cells tested are: Human dermal fibroblast, BJAB (human Burkitt lymphoma cell), Human iPS cell, NSC-34 (Mouse Motor Neuron Hybrid Cell), C2C12 cell (mouse myoblast), and HUVEC (Human Umbilical Vein Endothelial Cell)
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Product Sizes | 0.5 ml, 1.5 ml |
| Subcategories | Broad Spectrum |
Documents
Protocols
MSDS
Citations or Feedback
- Nasuhidehnavi, A., Zhao, Y., Punetha, A., Hemphill, A., Li, H., Bechtel, T. J., Rager, T., Xiong, B., Petrou, V. I., Gubbels, M.-J., Weerapana, E., & Yap, G. S. (2022). A role for Basigin in Toxoplasma gondii infection. Infection and Immunity, 90(6), Article e00205-22. https://doi.org/10.1128/iai.00205-22
