AsPC-1 Cell Avalanche® Transfection Reagent (human pancreas adenocarcinoma cell)

 
Description

Cell to transfect:

Designations: AsPC-1

Cell Synonyms: CRL-1682, pancreas, adenocarcinoma, human pancreas adenocarcinoma cell

Organism: Homo sapiens (human)

Tissue: adenocarcinoma; pancreas; derived from metastatic site: ascites

Age: 62 years

Gender: female

Ethnicity: Caucasian

Morphology: epithelial

Products: carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin

Growth Properties: adherent

Depositors: M.H. Tan

The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in AsPC-1 Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Capan-1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Capan-1 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on AsPC-1 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as AsPC-1 Cell Avalanche Transfection Reagent.

For Other Cells

AsPC-1 Cell Avalanche® Transfection Reagent (human pancreas adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

PANC-1 Cell

BxPC-3 Cell

293 Cell

293T/17 Cell

SK-OV-3 Cell

U-2 OS cell

Saos-2 Cell

SK-N-SH Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-ASPC-1 Protocol.

MSDS

EZT-ASPC-1 MSDS

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