AGS Cell Avalanche® Transfection Reagent (human gastric adenocarcinoma cell)

 
Description

Cell to transfect:

Designations: AGS

Cell Synonyms: CRL-1739, human gastric adenocarcinoma cell

Depositors: SC Barranco

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: stomach

Disease: gastric adenocarcinoma

Isolation date: 1979

Applications: transfection host

Tumorigenic: Yes

Age: 54 years

Gender: female

Ethnicity: Caucasian

Comments: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in AGS cells.
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Compatible with serum
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on AGS cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for AGS cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this AGS Cell Avalanche Transfection Reagent.

For Other Cells

AGS Cell Avalanche® Transfection Reagent (human gastric adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

NCI-N87 Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

PC-3 Cell

AR42J Cell

BxPC-3 Cell

AsPC-1 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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