EZT-A431-1 Protocol.
Description
Cell to transfect:
Designations: A-431
Cell Synonyms: : CRL-1555, human epidermoid carcinoma cell
Depositors: DJ Giard, SA Aaronson
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: skin
Tissue: epidermis
Disease: epidermoid carcinoma
Applications: transfection host
Tumorigenic: Yes
Age: 85 years
Gender: female
Comments: The epidermoid carcinoma cell line A-431, derived from an 85-year-old female, is one of a series of cell lines established from solid tumors by D.J. Giard, et al.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Unique formulation-maximize transfection performance in A-431 cells.
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Compatible with serum
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A431 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A431 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this A431 Cell Avalanche Transfection Reagent.
For Other Cells
A-431 Cell Avalanche® Transfection Reagent (human epidermoid carcinoma cell) can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
DU 145 Cell
22Rv1 Cell
VCaP Cell
PC-3 Cell
AR42J Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype | Adherent |
| Cell Type | Epithelial Cell |
| Disease | Cancer |
| Names starting from | A |
| Primary/Cell Line | Cell Line |
| Product Sizes | 0.5 ml, 1.5 ml |
| Species | Human |
| Tissue Sources | Skin |
| Subcategories | Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
- Wang, S., Allen, N., Vickers, T. A., Revenko, A. S., Sun, H., Liang, X. H., & Crooke, S. T. (2018). Cellular uptake mediated by epidermal growth factor receptor facilitates the intracellular activity of phosphorothioate-modified antisense oligonucleotides. Nucleic Acids Res, 46(7), 3579-3594. doi:10.1093/nar/gky145
