3T3-Swiss albino Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 3T3-Swiss albino cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 3T3-Swiss albino cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Cellular Products: Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway [26623]
Isolation date: 1962
Age: embryo
Comments: The 3T3 cell line was established by G. Todaro and H. Green in 1962 from disaggregated Swiss mouse embryos. The cells are contact inhibited.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in 3T3-Swiss albino Cells.
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3T3-Swiss albino cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3T3-Swiss albino Cell Avalanche Transfection Reagent.
FIG. 2. 3T3-Swiss albino cells were transfected with GFP vector (pEGFP-N3) by using 3T3-Swiss albino Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
3T3-Swiss albino Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
3197-3 Cell
NIH/3T3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
WI-38 Cell
MRC-5 Cell
IMR-90 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Cellular Products: Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway [26623]
Isolation date: 1962
Age: embryo
Comments: The 3T3 cell line was established by G. Todaro and H. Green in 1962 from disaggregated Swiss mouse embryos. The cells are contact inhibited.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in 3T3-Swiss albino Cells.
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3T3-Swiss albino cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3T3-Swiss albino Cell Avalanche Transfection Reagent.
FIG. 2. 3T3-Swiss albino cells were transfected with GFP vector (pEGFP-N3) by using 3T3-Swiss albino Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
3T3-Swiss albino Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
3197-3 Cell
NIH/3T3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
WI-38 Cell
MRC-5 Cell
IMR-90 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3T3-Swiss albino cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3T3-Swiss albino Cell Avalanche Transfection Reagent.
