3T3-L1 Cell Avalanche® Transfection Reagent

3T3-L1 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 3T3-L1 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 3T3-L1 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

$714.00$1,264.00

& Free Shipping

$714.00$1,264.00

 
Description

Cell to transfect:

Designations: 3T3-L1

Cell Synonyms: : CL-173

Depositors: Massachusetts Institute of Technology

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: embryo

Cell Type: fibroblast

Cellular Products: triglycerides

Applications: transfection host

Receptors: insulin, expressed

Age: embryo

L1 is a continuous substrain of 3T3 (Swiss albino) developed through clonal isolation. The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation [PubMed ID: 4426090].

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into 3T3-L1 cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3T3-L1 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3T3-L1 Cell Avalanche Transfection Reagent.

FIG. 2. 3T3-L1 cells were transfected with GFP vector (pEGFP-N3) by using 3T3-L1 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells

3T3-L1 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

NIH/3T3 Cell

BALB/3T3 clone A31

3T3-Swiss albino Cell

MDCK Cell

Vero Cell

293 Cell

MRC-5 Cell

IMR-90 Cell

COS-7 Cell

BHK-21 Cell

3197-3 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Fibroblast

Disease

Healthy

Names starting from

0-9

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Mouse

Tissue Sources

Embryo

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

 
Description

Cell to transfect:

Designations: 3T3-L1

Cell Synonyms: : CL-173

Depositors: Massachusetts Institute of Technology

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: embryo

Cell Type: fibroblast

Cellular Products: triglycerides

Applications: transfection host

Receptors: insulin, expressed

Age: embryo

L1 is a continuous substrain of 3T3 (Swiss albino) developed through clonal isolation. The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation [PubMed ID: 4426090].

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into 3T3-L1 cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3T3-L1 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3T3-L1 Cell Avalanche Transfection Reagent.

FIG. 2. 3T3-L1 cells were transfected with GFP vector (pEGFP-N3) by using 3T3-L1 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells

3T3-L1 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

NIH/3T3 Cell

BALB/3T3 clone A31

3T3-Swiss albino Cell

MDCK Cell

Vero Cell

293 Cell

MRC-5 Cell

IMR-90 Cell

COS-7 Cell

BHK-21 Cell

3197-3 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Fibroblast

Disease

Healthy

Names starting from

0-9

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Mouse

Tissue Sources

Embryo

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

Shopping Cart
Scroll to Top