3197-3 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on 3197-3 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 3197-3 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of 3197-3 Cell Avalanche® Transfection Reagent for transfection on 3197-3 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus. They release a growth factor in serum free medium. They do not produce virus particles. The cells have a tendency to slough off the flask surface. Tested and found negative for ectromelia virus (mousepox).
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in 3197-3 cells.
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3197-3 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3197-3 Cell Avalanche Transfection Reagent.
FIG. 2. 3197-3 cells were transfected with GFP vector (pEGFP-N3) by using 3197-3 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
3197-3 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
NIH/3T3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
3T3-Swiss albino Cell
786-O Cell
Caki-1 Cell
Detroit 551 Cell
MG-63 Cell
WI-38 Cell
MRC-5 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus. They release a growth factor in serum free medium. They do not produce virus particles. The cells have a tendency to slough off the flask surface. Tested and found negative for ectromelia virus (mousepox).
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in 3197-3 cells.
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3197-3 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3197-3 Cell Avalanche Transfection Reagent.
FIG. 2. 3197-3 cells were transfected with GFP vector (pEGFP-N3) by using 3197-3 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
3197-3 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
NIH/3T3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
3T3-Swiss albino Cell
786-O Cell
Caki-1 Cell
Detroit 551 Cell
MG-63 Cell
WI-38 Cell
MRC-5 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3197-3 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3197-3 Cell Avalanche Transfection Reagent.
