293 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on 293 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 293 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of 293 Cell Avalanche® Transfection Reagent for transfection on 293 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Comments: The cells containes Adenovirus 5 DNA from left ends of the viral genome. The line is excellent for titrating human adenoviruses. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit. The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2)
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into 293 cells
Highest efficiency to ensure experimental success
0.5 ml is able to transfect about 1000 wells of 24-well plate
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
Deliver single or multiple plasmids
Compatible with serum
Suitable for Reverse Transfection
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on 293 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for 293 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this 293 Cell Avalanche Transfection Reagent.
FIG. 2. 293 cells were transfected with GFP vector (pEGFP-N3) by using 293 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
293 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
Weight
0.5 lbs
Adherence Phenotype
Adherent
Cell Type
Epithelial Cell
Disease
Healthy
Names starting from
0-9
Primary/Cell Line
Cell Line
Product Sizes
0.5 ml, 1.5 ml
Species
Human
Tissue Sources
Kidney
Subcategories
Cell Type/Cell Line Specific
Documents
Protocols
EZT-2930-1 Protocol.
MSDS
EZT-2930-1 MSDS
Citations or Feedback
Amini, M., Wang, H., Belkacemi, A., Jung, M., Bertl, A., Schlenstedt, G., . . . Beck, A. (2019). Identification of Inhibitory Ca(2+) Binding Sites in the Upper Vestibule of the Yeast Vacuolar TRP Channel. iScience, 11, 1-12. doi:10.1016/j.isci.2018.11.037
Comments: The cells containes Adenovirus 5 DNA from left ends of the viral genome. The line is excellent for titrating human adenoviruses. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit. The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2)
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into 293 cells
Highest efficiency to ensure experimental success
0.5 ml is able to transfect about 1000 wells of 24-well plate
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
Deliver single or multiple plasmids
Compatible with serum
Suitable for Reverse Transfection
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on 293 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for 293 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this 293 Cell Avalanche Transfection Reagent.
FIG. 2. 293 cells were transfected with GFP vector (pEGFP-N3) by using 293 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
293 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
Weight
0.5 lbs
Adherence Phenotype
Adherent
Cell Type
Epithelial Cell
Disease
Healthy
Names starting from
0-9
Primary/Cell Line
Cell Line
Product Sizes
0.5 ml, 1.5 ml
Species
Human
Tissue Sources
Kidney
Subcategories
Cell Type/Cell Line Specific
Documents
Protocols
EZT-2930-1 Protocol.
MSDS
EZT-2930-1 MSDS
Citations or Feedback
Amini, M., Wang, H., Belkacemi, A., Jung, M., Bertl, A., Schlenstedt, G., . . . Beck, A. (2019). Identification of Inhibitory Ca(2+) Binding Sites in the Upper Vestibule of the Yeast Vacuolar TRP Channel. iScience, 11, 1-12. doi:10.1016/j.isci.2018.11.037
EZT-2930-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on 293 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for 293 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this 293 Cell Avalanche Transfection Reagent.
