COLO 205 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell)

Transfection of suspension/adherent mixed cells, such as COLO 205 cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

COLO 205 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting COLO 205 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for COLO 205 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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$714.00$1,264.00

 
Description

Cell to transfect:

Designations: COLO 205

Cell Synonyms: : CCL-222, Colo, NCI60, NCI-60, human colon adenocarcinoma cell

Depositors: GE Moore

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens

Morphology:epithelial

Source: Organ: colon

Tumor Stage: Dukes’ type D

Disease: colorectal adenocarcinoma

Derived from metastatic site: ascites

Cellular Products: carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10)

Applications: transfection host

Tumorigenic: Yes

Age: 70 years

Gender: male

Ethnicity: Caucasian

Comments: This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The cells are positive for keratin by imunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.

COLO 205 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into COLO 205 Cells
  • Highest efficiency to ensure experimental success
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on COLO 205 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for COLO 205 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this COLO 205 Cell Avalanche® Transfection Reagent.

For Other Cells

COLO 205 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype Mixed
Cell Type Epithelial Cell
Disease Cancer
Names starting from C
Primary/Cell Line Cell Line
Product Sizes 0.5 ml, 1.5 ml
Species Human
Tissue Sources Colon
Subcategories Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

 
Description

Cell to transfect:

Designations: COLO 205

Cell Synonyms: : CCL-222, Colo, NCI60, NCI-60, human colon adenocarcinoma cell

Depositors: GE Moore

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens

Morphology:epithelial

Source: Organ: colon

Tumor Stage: Dukes’ type D

Disease: colorectal adenocarcinoma

Derived from metastatic site: ascites

Cellular Products: carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10)

Applications: transfection host

Tumorigenic: Yes

Age: 70 years

Gender: male

Ethnicity: Caucasian

Comments: This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The cells are positive for keratin by imunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.

COLO 205 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into COLO 205 Cells
  • Highest efficiency to ensure experimental success
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on COLO 205 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for COLO 205 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this COLO 205 Cell Avalanche® Transfection Reagent.

For Other Cells

COLO 205 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype Mixed
Cell Type Epithelial Cell
Disease Cancer
Names starting from C
Primary/Cell Line Cell Line
Product Sizes 0.5 ml, 1.5 ml
Species Human
Tissue Sources Colon
Subcategories Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

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