HT-29 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell)

 
Description

Cell to transfect:

Designations: HT-29

Cell Synonyms: : HTB-38, colon carcinoma, cancer, NCI60, NCI-60, human colon adenocarcinoma cell

Depositors: J Fogh

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: colon

Disease: colorectal adenocarcinoma

Isolation date: 1964

Applications: transfection host

Receptors: human adrenergic alpha2A [23560], urokinase receptor (u-PAR), vitamin D (moderate expression), vitamin D (moderate expression).

Tumorigenic: Yes

Age: 44 years adult

Gender: female

Ethnicity: Caucasian

Comments: Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.

HT-29 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into HT-29 cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T84 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T84 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on HT-29 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as HT-29 Cell Avalanche Transfection Reagent.

For Other Cells

HT-29 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

T84 Cell

HCT 116 Cell

DLD-1 Cell

Caco-2 Cell

LS 174T Cell

LoVo Cell

SW480 Cell

SW620 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-HT29-1 Protocol.

MSDS

EZT-HT29-1 MSDS

Citations or Feedback

• Niemann, B., Nemitz, A., Werner, J., Mai, H. D., Steinberg, P., Lampen, A., & Ehlers, A. (2017). Folic acid modulates cancer-associated micro RNAs and inflammatory mediators in neoplastic and non-neoplastic colonic cells in a different way. Mol Nutr Food Res, 61(12). doi:10.1002/mnfr.201700260