NTERA-2 cl.D1 Cell Avalanche® Transfection Reagent (human testis carcinoma cell)

Name: NTERA-2 cl.D1 Cell Avalanche® Transfection Reagent (human testis carcinoma cell)
SKU: EZT-NTER-1
Availability: InStock

Transfection of pluripotent stem cells or stem-like cells, such as NTERA-2 cl.D1 cell line, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

NTERA-2 cl.D1 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on NTERA-2 cl.D1 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NTERA-2 cl.D1 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of NTERA-2 cl.D1 Cell Avalanche® Transfection Reagent for transfection on NTERA-2 cl.D1 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

$714.00 – $1,264.00

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SKU: EZT-NTER-1 Categories: All Cell Lines, Cell Type/Cell Line-specific Transfection Reagent, For Cell Lines, K-O, Transfection Reagents Tags: CRL-1973, epithelial-like, human testis carcinoma cell, lung, malignant pluripotent embryonal carcinoma, NT2/D1, NTERA-2 cl.D1, PW Andrews, testis

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$714.00 – $1,264.00

Description

Cell to transfect:

Designations: NTERA-2 cl.D1 [NT2/D1]

Cell Synonyms: : NT2/D1, CRL-1973. human testis carcinoma cell

Depositors: PW Andrews

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial-like, differentiation changes phenotype

Source: Organ: testis

Disease: malignant pluripotent embryonal carcinoma

Derived from metastatic site: lung

Applications: transfection host

Virus Resistance: UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)

Age: 22 years

Gender: male

Ethnicity: Caucasian

Comments: The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line. The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line. This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide ((HMBA). The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters. The cells exhibit high expression of N-myc oncogene activity.

Features of The Transfection Reagent:

Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into NTERA-2 cl.D1 cells
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NTERA-2 CL.D1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NTERA-2 CL.D1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NTERA-2 CL.D1 Cell Avalanche Transfection Reagent.

For Other Cells

NTERA-2 cl.D1 Cell Avalanche® Transfection Reagent (human testis carcinoma cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

DU 145 Cell

22Rv1 Cell

VCaP Cell

PC-3 Cell

AR42J Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight	0.5 lbs
Adherence Phenotype	Adherent
Cell Type	Epithelial Cell
Disease	Cancer
Names starting from	N
Primary/Cell Line	Cell Line
Product Sizes	0.5 ml, 1.5 ml
Species	Human
Tissue Sources	testes
Subcategories	Cell Type/Cell Line Specific

Documents

Protocols

EZT-NTER-1 Protocol.

MSDS

EZT-NTER-1 MSDS

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