U-87 MG Cell Avalanche® Transfection Reagent (human glioblastoma and astrocytoma cell)

 
Description

Cell to transfect:

Designations: U-87 MG

Cell Synonyms: HTB-14, human glioblastoma and astrocytoma cell

Depositors: J Ponten

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: brain

Tumor Stage: classified as grade IV as of 2007

Disease: glioblastoma; astrocytoma

Tumorigenic: Yes

Antigen Expression: Blood Type A, Rh+

Age: 44 years

Gender: male

Ethnicity: Caucasian

Comments: This is one of a number of cell lines derived from malignant gliomas by J. Ponten and associates from 1966 to 1969.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in U-87 MG cell.
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Animal-free
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on U-87 MG cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for U-87 MG cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this U-87 MG Cell Avalanche Transfection Reagent.

FIG. 2. U-87 cells were transfected with GFP vector (pEGFP-N3) by using U-87 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells

U-87 MG Cell Avalanche® Transfection Reagent (human glioblastoma and astrocytoma cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-U87M-1 Protocol.

MSDS

EZT-U87M-1 MSDS

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