IMR-90 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on IMR-90 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for IMR-90 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of IMR-90 Cell Avalanche® Transfection Reagent for transfection on IMR-90 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Cytogenetic Analysis: normal human female; diploid; stable
Isoenzymes: G6PD, B
Age: 16 weeks gestation
Gender: female
Ethnicity: Caucasian
The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. The line may be considered as an alternate for WI-38 and other standard human lung cell strains. The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into IMR-90 cells
Highest efficiency to ensure experimental success
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Compatible with serum an
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on WI-38 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for WI-38 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in IMR-90 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as IMR-90 Cell Avalanche Transfection Reagent.
FIG. 2. IMR-90 cells were transfected with GFP vector (pEGFP-N3) by using IMR-90 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
IMR-90 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Caki-1 Cell
MDCK Cell
293 Cell
WI-38 Cell
MRC-5 Cell
COS-7 Cell
BHK-21 Cell
3197-3 Cell
NIH/3T3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Cytogenetic Analysis: normal human female; diploid; stable
Isoenzymes: G6PD, B
Age: 16 weeks gestation
Gender: female
Ethnicity: Caucasian
The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. The line may be considered as an alternate for WI-38 and other standard human lung cell strains. The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into IMR-90 cells
Highest efficiency to ensure experimental success
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Compatible with serum an
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on WI-38 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for WI-38 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in IMR-90 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as IMR-90 Cell Avalanche Transfection Reagent.
FIG. 2. IMR-90 cells were transfected with GFP vector (pEGFP-N3) by using IMR-90 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
IMR-90 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Caki-1 Cell
MDCK Cell
293 Cell
WI-38 Cell
MRC-5 Cell
COS-7 Cell
BHK-21 Cell
3197-3 Cell
NIH/3T3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
EZT-IMR9-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on WI-38 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for WI-38 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in IMR-90 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as IMR-90 Cell Avalanche Transfection Reagent.
