WI-38 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on WI-38 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for WI-38 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of WI-38 Cell Avalanche® Transfection Reagent for transfection on WI-38 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue. WI-38 cells have a finite lifetime of 50 plus or minus 10 population doublings with a doubling time of 24 hours. This line was the first human diploid cell line to be used in human vaccine preparation.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in WI-38 cells.
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Compatible with transfection in any plate formats
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on WI-38 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for WI-38 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this WI-38 Cell Avalanche Transfection Reagent.
FIG. 2. WI-38 cells were transfected with GFP vector (pEGFP-N3) by using WI-38 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
WI-38 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
MRC-5 Cell
IMR-90 Cell
COS-7 Cell
BHK-21 Cell
3197-3 Cell
NIH/3T3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
Weight
0.5 lbs
Adherence Phenotype
Adherent
Cell Type
Fibroblast
Disease
Healthy
Names starting from
W
Primary/Cell Line
Cell Line
Product Sizes
0.5 ml, 1.5 ml
Species
Human
Tissue Sources
Lung
Subcategories
Cell Type/Cell Line Specific
Documents
Protocols
EZT-WI38-1 Protocol.
MSDS
EZT-WI38-1 MSDS
Citations or Feedback
Milewska, M., & Kolch, W. (2014). Mig-6 participates in the regulation of cell senescence and retinoblastoma protein phosphorylation. Cell Signal, 26(9), 1870-1877. doi:10.1016/j.cellsig.2014.05.003
The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue. WI-38 cells have a finite lifetime of 50 plus or minus 10 population doublings with a doubling time of 24 hours. This line was the first human diploid cell line to be used in human vaccine preparation.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in WI-38 cells.
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Compatible with transfection in any plate formats
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on WI-38 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for WI-38 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this WI-38 Cell Avalanche Transfection Reagent.
FIG. 2. WI-38 cells were transfected with GFP vector (pEGFP-N3) by using WI-38 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
WI-38 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
MRC-5 Cell
IMR-90 Cell
COS-7 Cell
BHK-21 Cell
3197-3 Cell
NIH/3T3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
Weight
0.5 lbs
Adherence Phenotype
Adherent
Cell Type
Fibroblast
Disease
Healthy
Names starting from
W
Primary/Cell Line
Cell Line
Product Sizes
0.5 ml, 1.5 ml
Species
Human
Tissue Sources
Lung
Subcategories
Cell Type/Cell Line Specific
Documents
Protocols
EZT-WI38-1 Protocol.
MSDS
EZT-WI38-1 MSDS
Citations or Feedback
Milewska, M., & Kolch, W. (2014). Mig-6 participates in the regulation of cell senescence and retinoblastoma protein phosphorylation. Cell Signal, 26(9), 1870-1877. doi:10.1016/j.cellsig.2014.05.003
EZT-WI38-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on WI-38 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for WI-38 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this WI-38 Cell Avalanche Transfection Reagent.
