Description
Cell to transfect:
Designations: 4T1
Cell Synonyms: : CRL-2539, mouse breast cancer cell
Depositors: BA Pulaski
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: epithelial
Organ: mammary gland
Strain: BALB/cfC3H
Disease: tumor
Tumorigenic: Yes
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment. When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer. 4T1-induced tumors can also be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics. Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy than most tumor models.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into 4T1 cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on 4T1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for 4T1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this 4T1 Cell Avalanche Transfection Reagent.
FIG. 2. 4T1 cells were transfected with GFP vector (pEGFP-N3) by using 4T1 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
4T1 Cell Avalanche® Transfection Reagent (mouse breast cancer cell) can also be used on the following cells with high transfection efficiencies.
ZR-75-1 Cell
BT-20 Cell
MDA-MB-453 Cell
HCC1937 Cell
MDA-MB-468 Cell
SK-BR-3 Cell
MDA-MB-231 Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight |
0.5 lbs |
| Adherence Phenotype |
Adherent |
| Cell Type |
Epithelial Cell |
| Disease |
Cancer |
| Names starting from |
0-9 |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Mouse |
| Tissue Sources |
Breast/Mammary |
| Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
EZT-4T10-1 Protocol.
MSDS
EZT-4T10-1 MSDS
Citations or Feedback
- Nebot-Bral, L., Hollebecque, A., Yurchenko, A. A., de Forceville, L., Danjou, M., Jouniaux, J. M., . . . Kannouche, P. (2022). Overcoming resistance to alphaPD-1 of MMR-deficient tumors with high tumor-induced neutrophils levels by combination of alphaCTLA-4 and alphaPD-1 blockers. J Immunother Cancer, 10(7), 1-15. doi:10.1136/jitc-2022-005059
- Garcia-Areas, R., Libreros, S., Simoes, M., Castro-Silva, C., Gazaniga, N., Amat, S., . . . Iragavarpu-Charyulu, V. (2017). Suppression of tumor-derived Semaphorin 7A and genetic ablation of host- derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma. Int J Oncol, 51(5), 1395-1404. doi:10.3892/ijo.2017.4144
Description
Cell to transfect:
Designations: 4T1
Cell Synonyms: : CRL-2539, mouse breast cancer cell
Depositors: BA Pulaski
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: epithelial
Organ: mammary gland
Strain: BALB/cfC3H
Disease: tumor
Tumorigenic: Yes
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment. When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer. 4T1-induced tumors can also be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics. Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy than most tumor models.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into 4T1 cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on 4T1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for 4T1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this 4T1 Cell Avalanche Transfection Reagent.
FIG. 2. 4T1 cells were transfected with GFP vector (pEGFP-N3) by using 4T1 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
4T1 Cell Avalanche® Transfection Reagent (mouse breast cancer cell) can also be used on the following cells with high transfection efficiencies.
ZR-75-1 Cell
BT-20 Cell
MDA-MB-453 Cell
HCC1937 Cell
MDA-MB-468 Cell
SK-BR-3 Cell
MDA-MB-231 Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight |
0.5 lbs |
| Adherence Phenotype |
Adherent |
| Cell Type |
Epithelial Cell |
| Disease |
Cancer |
| Names starting from |
0-9 |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Mouse |
| Tissue Sources |
Breast/Mammary |
| Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
EZT-4T10-1 Protocol.
MSDS
EZT-4T10-1 MSDS
Citations or Feedback
- Nebot-Bral, L., Hollebecque, A., Yurchenko, A. A., de Forceville, L., Danjou, M., Jouniaux, J. M., . . . Kannouche, P. (2022). Overcoming resistance to alphaPD-1 of MMR-deficient tumors with high tumor-induced neutrophils levels by combination of alphaCTLA-4 and alphaPD-1 blockers. J Immunother Cancer, 10(7), 1-15. doi:10.1136/jitc-2022-005059
- Garcia-Areas, R., Libreros, S., Simoes, M., Castro-Silva, C., Gazaniga, N., Amat, S., . . . Iragavarpu-Charyulu, V. (2017). Suppression of tumor-derived Semaphorin 7A and genetic ablation of host- derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma. Int J Oncol, 51(5), 1395-1404. doi:10.3892/ijo.2017.4144
