MOLT-4 Cell Avalanche® Transfection Reagent (human leukemia cell)

Name: MOLT-4 Cell Avalanche® Transfection Reagent (human leukemia cell)
SKU: EZT-MOLT-1
Availability: InStock

Transfection of suspension cells, such as MOLT-4 cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

MOLT-4 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on MOLT-4 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MOLT-4 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MOLT-4 Cell Avalanche® Transfection Reagent for transfection on MOLT-4 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

$714.00 – $1,264.00

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SKU: EZT-MOLT-1 Categories: All Cell Lines, Cell Type/Cell Line-specific Transfection Reagent, For Cell Lines, K-O, Transfection Reagents Tags: acute lymphoblastic leukemia, CRL-1582, human leukemia cell, lymphoblast, MOLT-4, NCI-60, NCI60, suspension, t lymphoblast

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$714.00 – $1,264.00

Description

Cell to transfect:

Designations: MOLT-4

Cell Synonyms: CRL-1582, NCI60, NCI-60, human leukemia cell

Organism: Homo sapiens (human)

Tissue: T lymphoblast; acute lymphoblastic leukemia

Age: 19 years

Gender: male

Morphology: lymphoblast

Growth properties: suspension

Tumorigenic: yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice.

Products: high levels of terminal deoxynucleotidyl transferase (TdT)

Depositor: J. Minowada

The line was established from cells taken from a patient in relapse. The patient had received prior multidrug chemotherapy.There is a G -> A mutation at codon 248 of the p53 gene. P53 is not expressed. The cells do not produce immunoglobulin or Epstein-Barr virus.

MOLT-4 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in MOLT-4 Cells.
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Animal-free
Compatible with serum
Compatible with transfection in any plate formats
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Developed and manufactured by EZ Biosystems, LLC

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MOLT-4 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MOLT-4 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MOLT-4 Cell Avalanche Transfection Reagent.

For Other Cells

MOLT-4 Cell Avalanche® Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies.

293 Cell

293T/17 Cell

NIH/3T3 Cell

3T3-L1 Cell

BALB/3T3 clone A31

3T3-Swiss albino Cell

CT26.WT Cell

NCTC Clone 929 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight	0.5 lbs
Adherence Phenotype	Suspension
Cell Type	lymphocyte
Disease	Cancer
Names starting from	M
Primary/Cell Line	Cell Line
Product Sizes	0.5 ml, 1.5 ml
Species	Human
Tissue Sources	Blood
Subcategories	Cell Type/Cell Line Specific

Documents

Protocols

EZT-MOLT-1 Protocol.

MSDS

EZT-MOLT-1 MSDS

Citations or Feedback

Abraham, B. J., Hnisz, D., Weintraub, A. S., Kwiatkowski, N., Li, C. H., Li, Z., . . . Young, R. A. (2017). Small genomic insertions form enhancers that misregulate oncogenes. Nat Commun, 8, 14385. doi:10.1038/ncomms14385
Hnisz, D., Weintraub, A. S., Day, D. S., Valton, A. L., Bak, R. O., Li, C. H., . . . Young, R. A. (2016). Activation of proto-oncogenes by disruption of chromosome neighborhoods. Science, 351(6280), 1454-1458. doi:10.1126/science.aad9024