EZT-SPAG-1 Protocol.
Description
Cell to transfect:
Designations: Sp2/0-Ag14
Cell Synonyms: SP20, Sp2/0, Ag14, CRL-1581, Sp2/Ag14, mouse hybridoma cell
Organism: Mus musculus mouse (B cell); mouse (myeloma)
Strain: BALB/c (B cell); BALB/c (myeloma)
Tissue: B lymphocyte; hybridoma
Morphology: lymphoblast
Growth properties: suspension
Depositor: G. Kohler
AntigenExp: H-2
The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 mcg/ml and are HAT sensitive. Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into Sp2/0-Ag14 cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SP2/0-AG14 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SP2/0-AG14 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SP2/0-AG14 Cell Avalanche Transfection Reagent.
For Other Cells
Sp2/0-Ag14 Cell Avalanche® Transfection Reagent (mouse hybridoma cell) can also be used on the following cells with high transfection efficiencies.
Vero Cell
293 Cell
293T/17 Cell
MG-63 Cell
WI-38 Cell
MRC-5 Cell
IMR-90 Cell
COS-7 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype | Suspension |
| Cell Type | lymphocyte |
| Disease | Cancer |
| Names starting from | S |
| Primary/Cell Line | Cell Line |
| Product Sizes | 0.5 ml, 1.5 ml |
| Species | Mouse |
| Tissue Sources | Spleen |
| Subcategories | Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
- Wieland, A., Kamphorst, A. O., Valanparambil, R. M., Han, J. H., Xu, X., Choudhury, B. P., & Ahmed, R. (2018). Enhancing FcgammaR-mediated antibody effector function during persistent viral infection. Sci Immunol, 3(27). doi:10.1126/sciimmunol.aao3125
