Saos-2 Cell Avalanche® Transfection Reagent (human osteosarcoma cell)

 
Description

Cell to transfect:

Designations: Saos-2

Cell Synonyms: HTB-85, osteosarcoma, human osteosarcoma cell

Organism: Homo sapiens (human)

Tissue: osteosarcoma; bone

Age: 11 years

Gender: female

Ethnicity: Caucasian

Morphology: epithelial

Growth properties: adherent

Tumorigenic: no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

AntigenExp: Blood Type B, Rh+; HLA A2, A3, Bw16, Bw47

Receptors expressed: epidermal growth factor (EGF); transforming growth factor beta (type 1 and type 2)

Depositors: J. Fogh; G. Trempe

This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh and G. Trempe.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in Saos-2 cells.
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SAOS-2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SAOS-2 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SAOS-2 Cell Avalanche Transfection Reagent.

FIG. 2. Saos-2 cells were transfected with GFP vector (pEGFP-N3) by using Saos-2 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells

Saos-2 Cell Avalanche® Transfection Reagent (human osteosarcoma cell) can also be used on the following cells with high transfection efficiencies.

U-2 OS cell

Vero Cell

293 Cell

786-O Cell

DU 145 Cell

22Rv1 Cell

VCaP Cell

PC-3 Cell

AR42J Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-SAOS-1 Protocol.

MSDS

EZT-SAOS-1 MSDS

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