Hs 578T Cell Avalanche® Transfection Reagent

 
Description

Cells to transfect:

Cell Line Name: Hs 578T

Cell Synonyms: ATCC HTB 126; ECACC 86082104; SPBIC, NCI60, NCI-60

Cell Synonyms: : ATCC HTB 126; ECACC 86082104; SPBIC.

Tissue: mammary gland; breast

Cell Type: fibroblast

Morphology: epithelial

Culture Properties: adherent

Mode of cultivation: monolayer

Tumorigenicity: tumorigenic in immunosupressed mice

Disease: ductal breast carcinoma

Age: 74 years adult

Gender: female

Ethnicity: Caucasian

Applications: antitumor tests, radiotherapy, tumorigenicity:

Comments:

The Hs 578T line was originated by A.J. Hackett, et al. along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient (J.Natl.Cancer Inst. 1977. 58: 1795-1806). The Hs 578T line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cloning. As with Hs 578Bst, no estrogen receptors or endogenous viruses were detected.

Hs 578T is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in Hs 578T cells
• Extremely gentle to cells
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Free of animal-derived components
• Active in serum-containing medium
• Suitable for Reverse Transfection
• Compatible with transfection in any plate format
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Great pricingDeveloped and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Hs 578T cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Hs 578T cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Hs 578T Cell Avalanche® Transfection Reagent.

FIG. 2. Hs 578T cells were transfected with GFP vector (pEGFP-N3) by using Hs 578T Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells

Hs 578T Cell Avalanche® Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies.

BT-20 Cell

HCC1937 Cell

MCF7 cell

T47D cell

MDA-MB-468 Cell

SK-BR-3 Cell

MDA-MB-231 Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-HS57-1 Protocol.

MSDS

EZT-HS57-1 MSDS

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