EZT-H226-1 Protocol.
Description
Cells to transfect:
Cell Line Name: NCI-H226 [H226] (ATCC® CRL-5826®)
Cell Synonyms: NCI60, NCI-60, H226, CRL-5826®
Organism: Homo sapiens, human
Tissue: lung, pleural effusion
Morphology: Epithelial
Culture Properties: adherent
Biosafety Level: 1
Disease: squamous cell carcinoma; mesothelioma
Gender: male
Karyotype: modal number = 47; range = 32-88; del(p25)
Name of Depositor: AF Gazdar, JD Minna
Year of Origin: March, 1980
Institution: University of Texas Southwestern Medical Center
NCI-H226 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
Transfection reagent features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Unique formulation-maximize transfection performance in NCI-H226 cells
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Active in serum-containing medium
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-H226 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-H226 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-H226 Cell Avalanche® Transfection Reagent.
For Other Cells
NCI-H226 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.
HOP-92 Cell
NCI-H322M Cell
A549 Cell
NCI-H292 Cell
NCI-H460 Cell
NCI-H23 Cell
NCI-H1975 Cell
NCI-H441 Cell
293 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Epithelial Cell |
| Disease |
Cancer |
| Names starting from |
N |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Lung |
| Subcategories |
Cell Type/Cell Line Specific |
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