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ACHN Cell Avalanche® Transfection Reagent (human kidney adenocarcinoma cell)

Catalog Number:EZT-ACHN-1
Cell Synonyms:ATCC® CRL-1611, NCI60, NCI-60

Cell Line Information:
Cell Line Name: ACHN (ATCC® CRL-1611™)
Organism: Homo sapiens, human  
Tissue: kidney, pleural effusion
Product Format: frozen  
Morphology: epithelial  
Culture Properties: adherent  
Disease: renal cell adenocarcinoma  
Age: 22 years  
Gender: male  
Ethnicity: Caucasian  
Tumorigenic: Yes
Applications: ACHN may be of use for antiproliferative studies using human interferons or interferon inducers.
Comments: Growth is inhibited by human interferon [Hogan, T. F., ]. ACHN is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
ACHN Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on ACHN cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for ACHN cells. No other transfection reagents can match the efficiency, convenience, and gentleness of ACHN Cell Avalanche® Transfection Reagent for transfection on ACHN cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
 

Features:

  • Unique formulation-maximize transfection performance in ACHN cells
  • Extremely gentle to cells
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Simplest and straightforward protocols to Increase experimental throughput
  • Active in serum-containing medium
  • Suitable for Reverse Transfection  
  • Compatible with transfection in any plate format
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on ACHN cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for ACHN cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this ACHN Cell Avalanche® Transfection Reagent.

For Other Cells:
ACHN Cell Avalanche® Transfection Reagent (human kidney adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

A498 Cell  
RXF393 Cell  
SN12C Cell  
TK10 Cell  
UO31 Cell  
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  0.5 ml $546.00
  1.5 ml $954.00
Kit Contents
. ACHN Cell Avalanche® Transfection Reagent (human kidney adenocarcinoma cell)
. Transfection Protocols
Related Products
. Avalanche®-Omni Transfection Reagent
. Avalanche®-in vivo Transfection Reagent
Transfection Protocol
Will be provided with the purchase of the reagent.
Shipping and storage

Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).

The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.

Country of Origin
USA
EZ Biosystems LLC. All Right Reserved