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| Avalanche®-PRO Transfection Reagent |
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Catalog Number：EZT-PROT-1 |
| Cell Synonyms：293 cells, CHO cells |
Avalanche®-PRO Transfection Reagent is a proprietary, animal origin-free formulation of lipids and polymers for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins. Avalanche®-PRO Transfection Reagent allows the highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications, and is specifically formulated for use with suspension 293 and CHO derived cells, such as FreeStyle 293-F Cells (suspension human embryonal kidney cells, Life Technologies, Cat. no. R790-07), FreeStyle CHO-S Cells (suspension Chinese Hamster Ovary cells, Life Technologies, Cat. no. R800-07), and DG44 Cells (DHFR- suspension CHO cells).
Features:
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Highest recombinant protein yield
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Less Transfection Reagent needed
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Compatible with multiple CHO media formulations
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Proven Scalability
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Simplest transfection protocol
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Totally animal free
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Half cost and similar or higher performances per reacton as compared to FreeStyle™ Max Reagent.
In brief, Avalanche®-PRO Transfection Reagent is ideal for Biotherapeutic Protein Production
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| Data： |
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| Figure 1. Less transfection reagent is needed for Avalanche®-PRO Transfection Reagent to achieve higher performances as compared to FreeStyle Max Reagent. (A) FreeStyle 293-F Cells were cultured in 30 ml of FreeStyle 293 Expression Medium to a density of 1 x 106 cells/ml for the transfection of pCMV∙SPROT beta-gal as plasmid DNA. For Avalanche®-PRO Reagent transfection, DNA and Transfection Reagent used are 30 ¦Ìg and 14 ¦Ìl respectively; For FreeStyle Max Reagent transfection, DNA and Transfection Reagent used are 37.5¦Ìg and 37.5 ¦Ìl respectively according to the respective protocols. (B) FreeStyle™ CHO-S Cells were cultured in 30 ml of FreeStyle™ CHO Expression Medium to a density of 1 x 106 cells/ml for the transfection of human IgG plasmid DNA. For Avalanche®-PRO Reagent transfection, DNA and transfection reagent used were 30 ¦Ìg and 15 ¦Ìl respectively; For FreeStyle Max Reagent transfection, DNA and Transfection Reagent used are 37.5¦Ìg : 37.5 ¦Ìl respectively according to the respective protocols.
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| Figure 2. Avalanche®-PRO provides high performance across varied media formulations. FreeStyle™ CHO-S cells were adapted to 8 representative growth media including: ProCHO™5 medium (Lonza Inc.), PowerCHO 2 CD medium (Lonza Inc), HyCell* CHO Medium (Thermo Scientific), FreeStyle™ CHO Expression medium (Life Technologies), Cellvento™ CHO-100 (EMD Millipore ), BD Select™ CHO medium (BD Biosciences), BD Select™ CD1000 medium (BD Biosciences), and EX-CELL® CD CHO-2 Medium (Sigma-Aldrich). Cells were transfected with a human IgG plasmid DNA using Avalanche-PRO (reagent:DNA=0.5 ¦Ìl:1.0 ¦Ìg/ml culture), or FreeStyle Max (Life Technologies) (reagent:DNA=1.25 ¦Ìl:1.25 ¦Ìg/ml culture) transfection reagents according to the published protocol ). Transfections were performed in 24-well deep well shaker blocks using 1 x 106 cells/ml at the time of transfection. Human IgG was quantitated from day 6 clarified supernatants and. Error bars represent the standard deviation of triplicate wells. |
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| Figure 3. Avalanche®-PRO Transfection Reagent Scalability and Cost Saving as compared to FreeStyle Max Reagent. (A)Avalanche®-PRO transfections in culture volumes from 1 ml to 250 ml resulted in consistant Human IgG yield in terms of ¦Ìg/ml culture. (B) and (C): While the transfection performance of Avalanche®-PRO Transfection Reagent is similar to that of FreeStyle MAX Reagent, the cost of Avalanche®-PRO needed per reacton is only half of the cost of FreeStyle MAX Reagent needed. |
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| Research Use Only. Not for use in diagnostic or therapeutic procedures. |
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| Purchase This Product |
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| Kit Contents |
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Avalanche®-PRO Transfection Reagent |
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Transfection Protocols |
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| Transfection Protocol |
| Will be provided with the purchase of the reagent. |
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| Shipping and storage |
Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).
The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.
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