Cell Information:

Cementum, a mineralized tissue lining the surface of the tooth root, is required for formation of a functional periodontal ligament attachment during development. Additionally, during regeneration of tissues after disease, cementum is thought to play a critical role in the reparative process.

Cementoblasts play a central role in periodontal development and regeneration, and in particular the formation of root cementum. Cementoblasts were first isolated from OC-TAG transfenic mice in Martha Somerman lab in University of Michigan, Ann Arbor. These mice contain a construct that employed the SV-40 T antigen under the direction of the osteocalcin (OCN) promoter. In this situation, when cells are isolated from the tooth root surface of OC-TAG mice, only those cells that express osteocalcin (i.e., cementoblasts) are capable of surviving serial passages in vitro. The immortalized cementoblasts obtained in this manner were subcloned, and one clone was designated as OCCM-30. These cells were shown to exhibit the same properties as comparable cells found in vivo, i.e., they expressed high levels of cementum-associated markers, such as BSP and OCN, and promoted biomineralization in vitro and in vivo.

The Transfection Reagent:

Cementoblast (OCCM-30 cell) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Cementoblasts (OCCM-30 cells). The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Cementoblasts (OCCM-30 cells). No other transfection reagents can match the efficiency, convenience, and gentleness of Cementoblast (OCCM-30 cell) Avalanche™ Transfection Reagent for transfection on Cementoblasts (OCCM-30 cells). For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

1. Zhao H, Qin X, Zhang Q, Zhang X, Lin J, Ting K, Chen F. Nell-1-ΔE, a novel transcript of Nell-1, inhibits cell migration by interacting with enolase-1. J Cell Biochem. 2018 Jul;119(7):5725-5733. doi: 10.1002/jcb.26756. Epub 2018 Apr 16. PubMed PMID: 29388706.
2. Zhang YY, Huang YP, Zhao HX, Zhang T, Chen F, et al. Cementogenesis is inhibited under a mechanical static compressive force via Piezo1. Angle Orthod. 2017 Jul;87(4):618-624. PubMed PMID: 28418701.
3. Zhang YY, Zhao HX, Chen ZB, Lin JX, Liu Y. Osteoprotegerin Promotes Cementoblastic Activity of Murine Cementoblast Cell Line in vitro. Chin J Dent Res. 2016 Jun;19(2):103-8. PubMed PMID: 27379348.

Features:

• Unique formulation-maximize transfection performance in Cementoblasts (OCCM-30 cells). More than 90% transfection efficiency as indicated by GFP positive cells in flowcytometry analysis.
• Extremely gentle to cells
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed

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