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Recombinant Proteins
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Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent

Catalog Number:EZT-PRSC-1
Cell Synonyms:PrSC, Prostate Stromal Cell

Cell Information:

Prostate cells provide a glandular function in the body by generating fluid which serves several functions in reproduction.  

Prostates consist of glandular epithelium embedded in a fibro-muscular stroma. The secretory luminal layer of the epithelium is made up of tall columnar cells that are responsible for the production of PSA, PAP and human kallikrein-2 that are secreted as part of the seminal fluid into the glandular lumina.

Human Prostate Stromal Cells (PrSC) are necessary for the normal growth and survival of epithelial cells in the prostate. A number of findings have suggested interactions between PrSC and the epithelium as well as importance of the extracellular matrix in prostate development and differentiation; alterations of these interactions may lead to prostate cancer progression (Djakiew et al., 1990; Cunha et al., 1996, 2002). Stromal–epithelial interactions appear to be reciprocal, mediated by steroid hormones, soluble and insoluble factors and their receptors, and constituents of the extracellular matrix (Chung, 1993; Rowley, 1999).

PrSC can be used for the following applications:

  • Benign prostatic hyperplasia (BPH)
  • Prostitis
  • Prostate cancer
  • Procreation research
  • Drug discovery
  • Male prostate physiology

The Transfection Reagent:

Transfection of primary cells, such as PrSc, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on PrSc. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PrSc. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent for transfection on PrSc. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Yu Q, Gratzke C, Wang R, Li B, Kuppermann P, Herlemann A, Tamalunas A, Wang Y, Rutz B, Ciotkowska A, Wang X, Strittmatter F, Waidelich R, Stief CG, Hennenberg M. A NAV2729-sensitive mechanism promotes adrenergic smooth muscle contraction and growth of stromal cells in the human prostate. J Biol Chem. 2019 Aug 9;294(32):12231-12249. doi: 10.1074/jbc.RA119.007958. Epub 2019 Jun 26. PubMed PMID: 31243101; PubMed Central PMCID: PMC6690707.

Features:

  • Specifically optimized to deliver nucleic acids into PrSc
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases 
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Simplest and straightforward protocols
  • Suitable for Reverse Transfection 
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

 

Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human prostate stromal cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human prostate stromal cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Prostate Stromal Cell Avalanche Transfection Reagent.

For Other Cells:
Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  0.5 ml $546.00
  1.5 ml $954.00
Kit Contents
. Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent
. Transfection Protocols
Related Products
. Avalanche®-Omni Transfection Reagent
. Avalanche®-in vivo Transfection Reagent
Shipping and storage

Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).

The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.

Country of Origin
USA
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