Cell Information:

Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. HUVEC are responsive to cytokine stimulation in the expression of cell adhesion molecules. These cell systems are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, and fibrinolysis. HUVEC can be used in the following applications:

• Cardiovascular pharmaceutical development
• Vascular pathology
• Atherosclerosis
• Angiogenesis
• Inflammation
• Wound healing
• Oncology

The Transfection Reagent:

Transfection of primary cells, such as HUVEC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Umbilical Vein Endothelial Cell (HUVEC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HUVEC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HUVEC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Umbilical Vein Endothelial Cell (HUVEC) Avalanche™ Transfection Reagent for transfection on HUVEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HUVEC
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

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