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Recombinant Proteins
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Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent

Catalog Number:EZT-HEK0-1
Cell Synonyms:HEK, NHEK, human epidermal keratinocyte

Cell Information:

Human Epidermal Keratinocytes (HEK) are primary cells isolated from normal human neonatal foreskin or adult skin. HEK is a suitable in vitro model for dermal toxicology tests in screening new skin products, and for developing dermal skin substitutes. HEK has been used in the following applications:

  • Epidermal model generation
  • Skin cancer
  • Toxicity (skin absorption studies)
  • Differentiation models (Ca++ free medium needed)
  • Epidermal disease research
  • Dermatitis/eczema, psoriasis, sebaceous cysts, folliculitis, and impetigo
  • Dermal burns
  • Wound healing

The Transfection Reagent:

Transfection of primary cells, such as HEK, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HEK. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HEK. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent for transfection on HEK. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Specifically optimized to deliver nucleic acids into HEK
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
  • Suitable for Reverse Transfection 
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human epidermal keratinocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human epidermal keratinocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Epidermal Keratinocyte Avalanche Transfection Reagent.

For Other Cells:
Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

LNCaP Cell
DU 145 Cell
22Rv1 Cell
VCaP Cell
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  0.5 ml $595.00
  1.5 ml $1,054.00
Kit Contents
. Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent
. Transfection Protocols
Related Products
. Avalanche®-Omni Transfection Reagent
. Avalanche®-in vivo Transfection Reagent
Transfection Protocol
Will be provided with the purchase of the reagent.
Shipping and storage

Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).

The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.

Country of Origin
USA
EZ Biosystems LLC. All Right Reserved