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Human Artery Endothelial Cell Avalanche™ Transfection Reagent

Catalog Number:EZT-HAOE-1
Cell Synonyms:HAOEC, HBcAEC, HCtAEC, HCAEC, HITAEC, HPAEC, HScAEC, Aortic, Brachiocephalic, Carotid, Coronary, Internal Thoracic, Pulmonary, Subclavian

Cell Information:

Vascular endothelial cells line the entire circulatory system, from the heart to the smallest capillaries. These cells have very distinct and unique functions that are paramount to vascular biology. These cells also play a pivotal role in a diverse range of physiological activities, such as angiogenesis, forming the selective blood barrier, arterial diseases and cancer development.

The Transfection Reagent:

Transfection of primary cells, such as Human Artery Endothelial Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Artery Endothelial Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Artery Endothelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Artery Endothelial Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Artery Endothelial Cell Avalanche™ Transfection Reagent for transfection on Human Artery Endothelial Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Specifically optimized to deliver nucleic acids into Human Artery Endothelial Cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
  • Compatible with serum
  • Compatible with transfection in any plate formats
  • Developed and manufactured by EZ Biosystems 

The transfection reagent work best for the following Human Artery Endothelial cells:

  • Human Aortic Endothelial Cell,
  • Human Brachiocephalic Artery Endothelial Cell,
  • Human Carotid Artery Endothelial Cell,
  • Human Coronary Artery Endothelial Cell,
  • Human Internal Artery Thoracic Endothelial Cell,
  • Human Pulmonary Artery Endothelial Cell,
  • Human Subclavian Artery Endothelial Cell
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human aortic endothelial cell after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human aortic endothelial cell. This test result was also confirmed by transfecting other artery endothelial cells mentioned above. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Artery Endothelial Cell Avalanche Transfection Reagent.

For Other Cells:
Human Artery Endothelial Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

Human Umbilical Vein Endothelial Cell (HUVEC)
Human Umbilical Artery Endothelial Cell (HUAEC)
Human Dermal Microvascular Endothelial Cell (HMVEC)
Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC)
Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  0.5 ml $595.00
  1.5 ml $1,054.00
Kit Contents
. Human Artery Endothelial Cell Avalanche™ Transfection Reagent
. Transfection Protocols
Related Products
. Avalanche®-Omni Transfection Reagent
. Avalanche®-in vivo Transfection Reagent
Transfection Protocol
Will be provided with the purchase of the reagent.
Shipping and storage

Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).

The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.

Country of Origin
USA
EZ Biosystems LLC. All Right Reserved