Cell Information:

Airway epithelial cells are targets in asthma, viral infection and fibrotic lung disease. Airway epithelial cells include human Bronchial, Tracheal, and Small Airway Epithelial Cells. These cells have been used to study the regulation of intracellular pH, the stimulant effect of IL-1b on airway epithelial cell growth, and the effect of human rhinovirus infection in terms of upregulation of ICAM-1 expression on epithelial cells.

Applications:

• Asthma
• COPD
• Airborne viruses
• Allergies
• Inhalation of nanoparticles
• Cancer
• Cystic Fibrosis
• DoD studies

The Transfection Reagent:

Transfection of primary cells, such as Human Airway Epithelial Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Human Airway Epithelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Airway Epithelial Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent? series?

References

1. Oniki K, Nohara H, Nakashima R, Obata Y, Muto N, Sakamoto Y, Ueno-Shuto K, Imafuku T, Ishima Y, Watanabe H, Maruyama T, Otake K, Ogata Y, Suico MA, Kai H, Shuto T, Saruwatari J. The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties. Sci Rep. 2020 Apr 6;10(1):5973. doi: 10.1038/s41598-020-62872-5. PubMed PMID: 32249844; PubMed Central PMCID: PMC7136289.
2. Zhou Y, Li P, Goodwin AJ, Cook JA, Halushka PV, et al. Exosomes from endothelial progenitor cells improve outcomes of the lipopolysaccharide-induced acute lung injury. Crit Care. 2019 Feb 13;23(1):44. PubMed PMID: 30760290; PubMed Central PMCID: PMC6373158.

Features:

• Specifically optimized to deliver nucleic acids into Human Airway Epithelial Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

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