Categories
Transfection Reagents
  For Cell Lines
  Names starting from
  A  B  C  D  E  F  G  H  I  J  K  L  M  N  O  P  Q  R  S  T  U  V  W  X  Y  Z  0-9 
  For Primary Cells
    ·  Astrocyte
    ·  beta cell
    ·  Cardiac Cell
    ·  Cementoblast
    ·  Chondrocyte
    ·  Endothelial Cell
    ·  Epithelial Cell
    ·  Fibroblast
    ·  Glial
    ·  Hepatocyte
    ·  Keratinocyte
    ·  lymphocyte
    ·  Macrophage
    ·  Melanocyte
    ·  Monocyte
    ·  Myoblast
    ·  Neural Cell
    ·  Osteoblast
    ·  Other Immune Cells
    ·  Others
    ·  Preadipocyte
    ·  Skeletal Muscle Cell
    ·  Smooth Muscle Cell
    ·  Stem Cell
    ·  Stromal Cell
    ·  Synoviocyte
Recombinant Proteins
  Protein Families
4T1 Cell Avalanche™ Transfection Reagent (mouse breast cancer cell)

Catalog Number:EZT-4T10-1
Cell Synonyms:CRL-2539

Cell Line Information:

Designations: 4T1
Depositors: BA Pulaski
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: epithelial
Organ: mammary gland
Strain: BALB/cfC3H
Disease: tumor
Tumorigenic: Yes
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment. When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer. 4T1-induced tumors can also be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics. Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy than most tumor models.

The Transfection Reagent:

4T1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 4T1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 4T1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Garcia-Areas R, Libreros S, Simoes M, Castro-Silva C, Gazaniga N, et al. Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma. Int J Oncol. 2017 Nov;51(5):1395-1404. PubMed PMID: 29048670; PubMed Central PMCID: PMC5642386.

Features:

  • Specifically optimized to deliver nucleic acids into 4T1 Cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Compatible with serum
  • Suitable for Reverse Transfection 
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on 4T1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for 4T1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this 4T1 Cell Avalanche Transfection Reagent.
FIG. 2. 4T1 cells were transfected with GFP vector (pEGFP-N3) by using 4T1 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:
4T1 Cell Avalanche™ Transfection Reagent (mouse breast cancer cell) can also be used on the following cells with high transfection efficiencies.

ZR-75-1 Cell
BT-20 Cell
MDA-MB-453 Cell
HCC1937 Cell
MDA-MB-468 Cell
SK-BR-3 Cell
MDA-MB-231 Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  0.5 ml $455.00
  1.5 ml $795.00
Kit Contents
. 4T1 Cell Avalanche™ Transfection Reagent (mouse breast cancer cell)
. Transfection Protocols
Related Products
. Avalanche®-Omni Transfection Reagent
. Avalanche®-in vivo Transfection Reagent
Transfection Protocol
Will be provided with the purchase of the reagent.
Shipping and storage

Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).

The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.

Country of Origin
USA
EZ Biosystems LLC. All Right Reserved