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Hepa 1-6 Cell Avalanche™ Transfection Reagent (mouse hepatoma cell)

Catalog Number:EZT-HEPA-1
Cell Synonyms:CRL-1830

Cell Line Information:

Designations: Hepa 1-6
Depositors: GJ Darlington
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: liver
Strain: C57L
Disease: hepatoma
Cellular Products: albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase
Applications: transfection host
Comments: This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.

The Transfection Reagent:

Hepa 1-6 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Hepa 1-6 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Hepa 1-6 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?


Zhang L, Bernardo KD, Vickers TA, Tian J, Liang XH, Crooke ST. NAT10 and DDX21 Proteins Interact with RNase H1 and Affect the Performance of Phosphorothioate Oligonucleotides. Nucleic Acid Ther. 2022 Jul 18. doi: 10.1089/nat.2021.0107. Epub ahead of print. PMID: 35852833.


  • Specifically optimized to deliver nucleic acids into Hepa 1-6 Cells
  • Highest efficiency to ensure experimental success
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Efficient for plasmid DNA/siRNA co-transfection
  • Compatible with serum
  • Suitable for Reverse Transfection 
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HEPA 1-6 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HEPA 1-6 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HEPA 1-6 Cell Avalanche Transfection Reagent.
FIG. 2. Hepa 1-6 cells were transfected with GFP vector (pEGFP-N3) by using Hepa 1-6 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:
Hepa 1-6 Cell Avalanche™ Transfection Reagent (mouse hepatoma cell) can also be used on the following cells with high transfection efficiencies.

Hep G2 Cell
Hep 3B Cell
HepG2/C3A Cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
CHO-K1 Cell
SK-OV-3 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  0.5 ml $595.00
  1.5 ml $1,054.00
Kit Contents
. Hepa 1-6 Cell Avalanche™ Transfection Reagent (mouse hepatoma cell)
. Transfection Protocols
Related Products
. Avalanche®-Omni Transfection Reagent
. Avalanche®-in vivo Transfection Reagent
Transfection Protocol
Will be provided with the purchase of the reagent.
Shipping and storage

Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).

The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.

Country of Origin
EZ Biosystems LLC. All Right Reserved