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bEnd.3 Cell Avalanche™ Transfection Reagent (mouse brain endothelioma cell)

Catalog Number:EZT-BEND-1
Cell Synonyms:CRL-2299

Cell Line Information:

Designations: bEnd.3
Depositors: EC Butcher
Growth Properties: adherent
Organism: Mus musculus
Morphology: endothelial
Source: Organ: brain
Strain: BALB/c
Tissue: cerebral cortex
Disease: endothelioma
Cell Type: endothelialpolyoma middle T antigen transformed
Age: 6 weeks
Comments: The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen. The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL). The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.3 cells by cytokines and lipopolysaccharide (LPS). Intracellular adhesion molecule 1 (ICAM-1) is constitutively expressed on the cells, and expression is increased by treatment with LPS, IL-1 and TNF alpha. Vascular cell adhesion molecule 1 (VCAM-1) is constitutively expressed on the cells at early passages but not at passages over 30.

The Transfection Reagent:

bEnd.3 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on bEnd.3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for bEnd.3 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of bEnd.3 Cell Avalanche™ Transfection Reagent for transfection on bEnd.3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Sonar SA, Shaikh S, Joshi N, Atre AN, Lal G. IFN-γ promotes transendothelial migration of CD4+ T cells across the blood-brain barrier. Immunol Cell Biol. 2017 Oct;95(9):843-853. PubMed PMID: 28682305.

Features:

  • Unique formulation-maximize transfection performance in bEnd.3 Cells.
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on bEnd.3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for bEnd.3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this bEnd.3 Cell Avalanche Transfection Reagent.

For Other Cells:
bEnd.3 Cell Avalanche™ Transfection Reagent (mouse brain endothelioma cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  0.5 ml $546.00
  1.5 ml $954.00
Kit Contents
. bEnd.3 Cell Avalanche™ Transfection Reagent (mouse brain endothelioma cell)
. Transfection Protocols
Related Products
. Avalanche®-Omni Transfection Reagent
. Avalanche®-in vivo Transfection Reagent
Transfection Protocol
Will be provided with the purchase of the reagent.
Shipping and storage

Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).

The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.

Country of Origin
USA
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