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| RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell) |
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Catalog Number：EZT-RBL2-1 |
| Cell Synonyms：CRL-2256 |
Cell Line Information:
Designations: RBL-2H3
Depositors: RP Siraganian
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: fibroblast
Source: peripheral blood
Strain: Wistar
Disease: basophilic leukemia
Cell Type: basophil; chemically induced
Cellular Products: histamine
Applications: transfection host
Receptors: FcERI (Fc of IgE)
Comments: RBL-2H3 is a basophilic leukemia cell line isolated and cloned in 1978 from Wistar rat basophilic cells that were maintained as tumors.
These cells have high affinity IgE receptors. They can be activated to secrete histamine and other mediators by aggregation of these receptors or with calcium ionophores. They have been used extensively to study FcERI and the biochemical pathways for secretion in mast cells. They have also been used extensively for studies of different aspects of secretion in cells including the role of changes in intracellular calcium, the activation of phospholipases, protein kinases and small G proteins.
The Transfection Reagent:
RBL-2H3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RBL-2H3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RBL-2H3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
references:
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Suzuki Y, Liu S, Ogasawara T, Sawasaki T, Takasaki Y, Yorozuya T, Mogi M. A novel MRGPRX2-targeting antagonistic DNA aptamer inhibits histamine release and prevents mast cell-mediated anaphylaxis. Eur J Pharmacol. 2020 Jul 5;878:173104. doi: 10.1016/j.ejphar.2020.173104. Epub 2020 Apr 19. PubMed PMID: 32320700.
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Sahid MNA, Liu S, Mogi M, Maeyama K. Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3. Inflamm Res. 2020 Mar;69(3):289-298. doi: 10.1007/s00011-020-01319-z. Epub 2020 Jan 28. PubMed PMID: 31993675.
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Liu S, Sahid MN, Takemasa E, Kiyoi T, Kuno M, et al. CRACM3 regulates the stability of non-excitable exocytotic vesicle fusion pores in a Ca(2+)-independent manner via molecular interaction with syntaxin4. Sci Rep. 2016 Jun 15;6:28133. PubMed PMID: 27301714; PubMed Central PMCID: PMC4908399.
Features:
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Unique formulation-maximize transfection performance in RBL-2H3 Cells.
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Extremely gentle to cells
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0.5 ml is able to transfect about 1000 wells of 24-well plate
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Deliver single or multiple plasmids
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Compatible with serum
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Compatible with transfection in any plate formats
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Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
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Developed and manufactured by EZ Biosystems
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| Data： |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RBL-2H3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RBL-2H3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RBL-2H3 Cell Avalanche Transfection Reagent. |
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For Other Cells： |
RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell) can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
| Research Use Only. Not for use in diagnostic or therapeutic procedures. |
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| Purchase This Product |
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RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell) |
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Transfection Protocols |
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| Transfection Protocol |
| Will be provided with the purchase of the reagent. |
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| Shipping and storage |
Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days).
The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.
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