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RSPO-1 (R-Spondin 1), Mouse, With C-mFc Tag
Catalog Number: EZC-RS1-M1
Synonym: RSPO1, RSPO-1, CRISTIN3, CRISTIN-3, FLJ40906, RP11-566C13.1, RSPO, R-spondin-1, Rspondin-1, Rspondin1
Source: Recombinant mouse RSPO1 mFc Chimera (rmRSPO1-mFc), Met1-Asn 932 (Accession # P08069) was produced in human HEK293 cells.
Molecular Characterization: rmRSPO1-mFc was fused with the mouse Fc region of at the C-terminus, the reduced monomer has a calculated molecular mass of 58.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmRSPO1-mFc monomer is approximately 67-75kDa due to the glycosylation.
Purity: >95% as determined by SDS-PAGE. All lots are greater than 95% pure.
Endotoxin: Less than 1.0 EU per 1 g of the rmRSPO1-mFc by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rmRSPO1-mFc should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: Mouse R-Spondin 1 ( Roof plate-specific Spondin 1 ), also known as RSPO1 and CRISTIN3, FLJ40906 or RP11-566C13.1, Which is a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain, which belongs to the R-Spondin family. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. R-Spondin1 activity critically depends on the presence of canonical Wnt ligands and LRP6. R-Spondin1 does not directly activate LRP6, it interferes with DKK1 / Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 levels on the cell surface. Mouse R-Spondin1 shares 89%, 87%, 92%, 91%, 91%, and 89% aa identity with human R-Spondin1. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC)[1]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  50 g $490.00
  1 mg $3,024.00
Reference
(1) Parma, P. et al., 2006, Nature genetics. 38 (11): 1304-9 (2) Capel, B., 2006, Nature genetics. 38 (11) :1233-4 (3) Binnerts, ME. et al., 2007, Proc Natl Acad Sci. 104 (37): 14700-5 (4) Zhao, J. et al., 2009, Proc Natl Acad Sci. 106 (7) :2331-6
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