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BACE1, (Beta-secretase-1), Human, With C-His Tag
Catalog Number: EZC-BA1-H2
Synonym: BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, Beta-secretase-1, memapsin-2, aspartyl-protease-2, beta-site-APP-cleaving-enzyme-1
Source: Recombinant human BACE1 (rhBACE1) Met 1-Thr 457 (Accession # NP_036236.1 ) fused with a C-terminal 6his tag was produced in human HEK293 cells.
Molecular Characterization: rhBACE1 was fused with a C-terminal 6his tag and has a calculated MW of 49.2 kDa. The predicted N-terminal is Thr22 or Glu46 (rare). DTT-reduced protein migrates as 50-65 kDa polypeptide in SDS-PAGE due to different glycosylation.
Purity: >95% as determined by SDS-PAGE. All lots are greater than 95% pure.
Endotoxin: Less than 1.0 EU per 1 g of the rhBACE1 by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhBACE1 should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: Beta-secretase 1 (BACE1) also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), -Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells.[1] The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid- peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. [2-3].
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  100 g $470.00
  1 mg $2,400.00
Reference
(1) Willem M, et al., 2006, Science 314 (5799): 6646. (2) Zacchetti D, et al., 2007, Neurodegener Dis. 4: 117-126. (3) Zhao J. et al., 2007, J. Neurosci. 27: 3639- 3649.
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