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PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9), Human
Catalog Number: EZC-PC9-H1
Synonym: PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Source: Recombinant human PCSK9(rhPCSK9) Met1-Gln692 (Accession # AAI66619) was produced in human HEK293 cells.
Molecular Characterization: rhPCSK9 contains C-terminal polyhistidine tag and has a calculated MW of 75.1 kDa. As a result of glycosylation and proteolytic digestion, DTT-reduced protein migrates as 20 kDa and 62 kDa polypeptide in SDS-PAGE.
Purity: >97% as determined by SDS-PAGE. All lots are greater than 97% pure.
Biological Activity: Measure by its ability to bind with human LDLR in a functional ELISA Immobilized recombinant human PCSK9 at 10 g/mL (100 l/well) can bind biotinylated human LDLR.The ED50 for this effect is typically 15 ng/mL.
Endotoxin: Less than 1.0 EU per 1 g of the rhPCSK9 by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhPCSK9 should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons.(1) Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).(2,3).
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  50 g $650.00
  1 mg $4,520.00
Reference
(1) Seidah NG, et al., Proc. Natl. Acad. Sci. U.S.A. 100 (3): 92833. (2) Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156. (3) Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 14549.
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