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B7-2 / CD86, Human
Catalog Number: EZC-B72-H3
Synonym: CD86, CD-86, B7-2, B70, B-70, CD28LG2, LAB72, LAB-72, MGC34413, Cluster of Differentiation 86
Source: Recombinant human CD86 / B7-2 (rhCD86-Fc) Leu26-His245 (Accession # AAH40261) was produced in human HEK293 cells.
Molecular Characterization: rhCD86-Fc, fused with Fc fragment of human IgG1 at the C-terminal and has a calculated MW of 51.5 kDa expressed. The predicted N-terminal is Leu26. Protein migrates as 80-95 kDa in reduced SDS-PAGE due to glycosylation.
Purity: >96% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU per 1 g of the rhCD86-Fc by the LAL method.
Formulation: Supplied as a 0.22 m filtered solution in 50 mM tris, 100 mM glycine, pH7.0 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization. Reconstitution instruction or specific concentrations are included in the hardcopy of CoA along with the products.
Storage: No activity loss was observed after storage at 4-8? for 1 month or -20? for 1 year in lyophilized state. rhCD86-Fc should be aliquoted and stored at -80? in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles.
Background: Cluster of Differentiation 86 (CD86), also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily,[1] and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon ?. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival.[2] Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-?B-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.[3].
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  200 g $543.00
  1 mg $2,016.00
Reference
(1) Chen C, et al., 1994, J. Immunol. 152 (10): 492936. (2) Yadav, D. et al., 2007, J. Immunol. 178: 6236-6241. (3) Steidl C, et al., 2010, N. Engl. J. Med. 362 (10): 87585.
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